Biotransformation and Biocatalysis (Dr. Igor A. Parshikov)Edit
Some information about my researches and publications (Паршиков И.А.) in fields of biotechnology, biochemistry and medicinal chemistry in different universities of world.
The using of microbial enzymes in the area of chemical, biotechnological and pharmacological industries has been carried out for a long time. The hydroxylation of organic compounds represents a big practical interest, because it allows to receiving as well known and new medical drugs.
Until recently, the microbial oxydation of heterocyclic compounds was achieved very well. Thus the regio‑ and stereoselective hydroxylation of the alkyl substituent was reported. A hypothesis has been put forward that in most cases the distance of 0.55 nm is preserved between the polar amide group of the substrate and the site of its binding to the enzyme. It is interesting to check this hypothesis again by subjecting different substrates to the transformation by various fungi species. It is known that the hydroxylation of various organic substrates is performed by the cytochrome P‑450 dependent monooxygenases. It was shown with methylpyridines that the monooxygenase system of Beauveria bassiana is cytochrome P‑450 dependent.
Presently the ability of a given fungi strain to transform different substrates is practically unpredictable, so many of the researchers resort to the systematic screening of microorganisms. Development of the enzyme-substrate interaction models is therefore necessary to replace the ineffective screening by either the systematically directed modification of the substrate rendering it susceptible for a fungi strain with well established transforming abilities, or by the effective use of enzyme induction, which may enable the desired transformation even in the case where it does not occur without inductors.